| 过氧化物模拟酶Fe-TPPS_4-隐性结晶紫-H_2O_2体系的催化显色反应研究 |
| |
| 引用本文： | 汪安,吴小蔚,孙淑声,慈云祥. 过氧化物模拟酶Fe-TPPS_4-隐性结晶紫-H_2O_2体系的催化显色反应研究[J]. 北京大学学报(自然科学版), 1992, 0(4) |
| |
| 作者姓名： | 汪安 吴小蔚 孙淑声 慈云祥 |
| |
| 作者单位： | 化学系(汪安，吴小蔚，孙淑声)，化学系(慈云祥) |
| |
| 摘 要： | 本文研究了Fe(Ⅲ)与meso-(四(4-磺基苯)卟啉(TPPS_4)的螯合物(Fe-TPPS_4)作为过氧化物模拟酶催化H_2O_2氧化隐性结晶紫为结晶紫的显色体系。该体系用于测定H_2O_2时的摩尔吸光系数为3.8×10~4,工作曲线的相关系数为0.996。此体系可与葡萄糖氧化酶催化反应偶联,用标准加入法测定人血清中葡萄糖的含量。
|
| 关 键 词： | 模拟过氧化物酶 催化显色反应 葡萄糖测定 分光光度法 |
Catalytic Color Reaction of the System Mimetic Peroxidase (Fe-TPPS4) -Leuco Crystal Violet-Hydrogen Peroxide |
| |
| WANG An,WU Xiaowei,SUN Shusheng,CI Yunxiang. Catalytic Color Reaction of the System Mimetic Peroxidase (Fe-TPPS4) -Leuco Crystal Violet-Hydrogen Peroxide[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1992, 0(4) |
| |
| Authors: | WANG An WU Xiaowei SUN Shusheng CI Yunxiang |
| |
| Abstract: | The mimetic peroxidase (Fe-TPPS4) can be used as a catalyst in the spectrophoto-metric determination of H2O2 and glocose. The method is based on the formation of crystal violet from leuco crystal violet. The glucose in human serum can be determined by the reaction combined with the catalytic reaction of glucose oxidase. |
| |
| Keywords: | Mimetic enzyme Catalytic color reaction Determination of glucose Spectrophotometry |
| 本文献已被 CNKI 等数据库收录！ |
