| 新型Gd基T2造影剂的制备和应用 |
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| 引用本文： | 张艳辉,张宏岩,张海禄,张朋利,蒋海珍,邓宗武,谭波. 新型Gd基T2造影剂的制备和应用[J]. 波谱学杂志, 2017, 34(3): 302-310. DOI: 10.11938/cjmr20172558 |
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| 作者姓名： | 张艳辉 张宏岩 张海禄 张朋利 蒋海珍 邓宗武 谭波 |
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| 作者单位： | 1. 上海大学 理学院, 上海 200444;2. 中国科学院 苏州纳米技术与纳米仿生研究所, 江苏 苏州 215123 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 设计并合成了结构为TPP-Lys（Acp-DOTA-Gd）-COOH（简称Gd-DOTA-TPP）的小分子磁共振探针，通过电转染的方式用探针标记人源脐带间充质干细胞（hMSCs）.11.7 T磁共振成像（MRI）扫描结果表明，Gd-DOTA-TPP标记的hMSCs在细胞内Gd含量为9×109 Gd/cell时，T2加权信号强度即可低至背景信号强度，呈现较强暗信号.将Gd-DOTA-TPP标记的hMSCs移植入小鼠脑室，可明显提高移植干细胞在MRI设备上的检测灵敏度，检测限可低至103个细胞.
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| 关 键 词： | 造影剂 人源脐带间充质干细胞(hMSCs) 磁共振成像(MRI) 灵敏度 TPP-Lys (Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP) |
| 收稿时间： | 2017-02-04 |
A New Gd-Based T2-Weighted Magnetic Resonance Imaging Contrast Agent: Preparation and Application in Stem Cell Imaging |
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| ZHANG Yan-hui,ZHANG Hong-yan,ZHANG Hai-lu,ZHANG Peng-li,JIANG Hai-zhen,DENG Zong-wu,TAN Bo. A New Gd-Based T2-Weighted Magnetic Resonance Imaging Contrast Agent: Preparation and Application in Stem Cell Imaging[J]. Chinese Journal of Magnetic Resonance, 2017, 34(3): 302-310. DOI: 10.11938/cjmr20172558 |
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| Authors: | ZHANG Yan-hui ZHANG Hong-yan ZHANG Hai-lu ZHANG Peng-li JIANG Hai-zhen DENG Zong-wu TAN Bo |
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| Affiliation: | 1. College of Sciences, Shanghai University, Shanghai 200444, China;2. Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China |
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| Abstract: | We synthesized TPP-Lys(Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP) as a novel T2-weighted MRI contrast agent, and used it to label human mesenchymal stem cells (hMSCs) via electroporation. hMSCs labeled with Gd-DOTA-TPP presented a rather dark T2-weighted image at a cellular Gd content of about 9×109 Gd/cell. The labeled cells were syringe-injected into a mouse brain with defined cell numbers followed by T2-weighted MRI on an 11.7 T system, which yield an in vivo imaging sensitivity of about 103 cells. |
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| Keywords: | magnetic resonance imaging (MRI) contrast agent human mesenchymal stem cells (hMSCs) TPP-Lys(Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP) sensitivity |
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