Uricase-Based Highly Sensitive and Selective Spectrophotometric Determination of Uric Acid Using BSA-Stabilized Au Nanoclusters as Artificial Enzyme |
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Authors: | Huihui Zhao Zhonghui Wang Xue Jiao Lichun Zhang |
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Affiliation: | 1. Key Lab of Green Chemistry &2. Technology of MOE at College of Chemistry , Sichuan University , Chengdu , Sichuan , China |
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Abstract: | ABSTRACT The enzymatic spectrophotometric analysis of uric acid based on BSA-stabilized Au nanoclusters (Au NCs) as peroxidase mimetics was first developed. Compared with natural enzyme horseradish peroxidase (HRP), which was used widely to detect H2O2 generated by uric acid, the BSA-stabilized Au NCs as peroxidase mimetics are easy to prepare, low cost, and stable. Kinetic analysis indicates that the BSA-stabilized Au NCs have even higher catalytic activity than HRP. Under the optimum conditions, the detection limit for uric acid is 3.6 × 10?7 mol L?1. The feasibility of the developed method for uric acid analysis in human serum was confirmed. |
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Keywords: | au nanoclusters biological template peroxidase mimetics uric acid |
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