Undergraduate Analytical Chemistry: To Use and Evaluate Organic Chelators for Spectrophotometric Determination of Iron |
| |
| Authors: | Mark T. Stauffer |
| |
| Affiliation: | 1. Natural Sciences Division – Chemistry , University of Pittsburgh at Greensburg , Greensburg, Pennsylvania, USA mtschem1@pitt.edu |
| |
| Abstract: | Abstract An analytical chemistry laboratory project to use and evaluate pyridyl‐ and triazine‐containing chelators for spectrophotometric determination of iron is described. The “Iron Project” introduces students to UV‐visible spectrophotometry and Beer's law via hands‐on development and use of spectrophotometric methods for iron. Nine chelators are currently available for use. Students perform their proposed work, culminating in formal papers and posters. Student values of molar absorptivities (?max) and λmax for ligands 1–9 agree with published values to within ±15% (?max) and ±3 nm (λmax) for 75% of results obtained so far. Stoichiometries for Fe(II) chelates of ligands 1–5 are within ±1 mole of ligand of the published ratios. Student work shows a basic understanding of Beer's law as well as a need to improve writing, problem‐solving, and laboratory skills. |
| |
| Keywords: | Beer's law calibration chelate iron limit of detection limit of quantitation method of continuous variations pyridyl- and triazine-type ligands UV‐visible spectrophotometry |
|
|
