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Analyzing Increased Fluorescence of Single Quantum Dots on Dehydrated Agarose by Single-Molecular Imaging |
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| Authors: | Jianfeng Wang Wei Liu Baoquan Liu Shengdi Fan |
| Institution: | 1. Key Laboratory of Biotechnology &2. Bioresources Utilization of the State Ethnic Affairs Commission-Ministry of Education, School of Life Science, Dalian Nationalities University , Dalian , China;3. School of Pharmacy, Jiangsu University , Zhenjiang , China |
| Abstract: | A single-molecule imaging technique was used to study emission behaviors of quantum dots (QDs) on the surface of glass and dehydrated agarose. The luminescence intensity of single QDs on the dehydrated agarose film was 2 or 3 times that on the glass. Our results revealed that the roughness and the hydrophobic nature of dehydrated agarose gel can suppress the blinking of single QDs. A clear decrease in the power law exponent for “on” time but an increase for “off” time was observed when the glass was replaced with 4% and 8% dehydrated agarose. The enhanced luminescence of QDs on dehydrated agarose will make their applications on microarrays more attractive. |
| Keywords: | dehydrated agarose quantum dots single-molecular imaging |