In Situ Measurement of Excitation Intensity for Fluorescence Excitation Spectroscopy |
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Authors: | K. Razi Naqvi |
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Affiliation: | Department of Physics , Norwegian University of Science and Technology , N-7034, Trondheim, Norway |
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Abstract: | It is argued that the double-beam method for the automatic correction of fluorescence excitation spectra, which proved to be a great boon in the days of analog instrumentation, has become, after the advent of digital techniques for acquiring and manipulating spectral data, more of a hindrance than a help; the benefits of reverting to a single beam device and making in situ measurement of the excitation intensity are pointed out. |
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Keywords: | Fluorescence spectroscopy Fluorescence excitation spectroscopy Spectral correction method Photosynthetic specimens. |
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