Emodin,A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant |
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Authors: | Mohammed A. Al-Nuri Nidal A. Za'tar Maher A. Abu-Eid Mohammad A. Hannoun Waheed J. Al-Jondi Ahmad I. Hussein |
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Affiliation: | Chemistry Department , An-Najah N. University , Nablus, West Bank of Jordan |
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Abstract: | A new method for isolation and specrophotometric determination of emodin is presented. Emodin was isolated by thin layer chromatography (tlc) and column chromatography (cc) techniques, as an orange long crystalline substance. Emodin exhibits two absorption maxima, at 420 and 520 nm. Stability of the color and the effect of pH were studied. Beer's law is obeyed in the range 2–30 ppm. The method is applied to the determination of emodin in roots, stems, and leaves of Rumex cyprius plant. |
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Keywords: | Emodin Spectrophotometric Determination Isolation Rumex Cyprius |
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