|
|||||
|
|
Magnetic Circular Dichroism Study of Analogues of the DNA Binding Ligands Netropsin and Distamycin A |
|
| Authors: | Christoph Zimmer Christian Marck Wilhelm Guschlbauer |
| Affiliation: | 1. Akademie der Wissenschaften der DDR, Forschungszentrum für Molekularbiologie und experimentelle Therapie, Abteilung Biochemie , Beutenbergstrasse 11, DDR-69, Jena, G. D. R.;2. Service de Biochimie, Département de Biologie , Centre d'Etudes Nucléaires de Saclay , B. P. N°2, F-91190, Gif-sur-Yvette, France |
| Abstract: | UV absorption and MCD data of a complete series of netropsin-like oligopeptides containing between one and five N-methyl-pyrrole residues are reported. The presence of three MCD bands in the region between 220 and 350 nm can be related to the resonance of the peptide groups with the methylpyrrole systems of the oligopeptides. The longest wavelength absorption bands of all netropsin-like compounds investigated are ascribed to two electronic transitions of the resonance methypyrrole-carboxamide units. - Complex formation with DNA affects these MCD bands and hence modifies the transitions. This is explained by hypercon-jugation in the chromophoric system as a result of specific hydrogen bonding of peptide groups with dA·dT pairs of double stranded DNA. |
| Keywords: | |