Studies on the interaction of gliclazide with bovine serum albumin by fluorescence and synchronous fluorescence spectroscopy |
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Authors: | Qiu-Ju Zhang Rong Han Gai-Xia Li |
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Institution: | 1. Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry &2. Environmental Science, Hebei University, Baoding, Hebei Province, P. R. China |
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Abstract: | The interaction between gliclazide and bovine serum albumin was investigated by fluorescence and synchronous fluorescence spectroscopy. From the experimental results, it was found that the quenching mechanism was static. The results of the synchronous fluorescence obtained indicated that the binding of gliclazide with bovine serum albumin could affect conformation in bovine serum albumin. In addition, the binding constants (Ka), binding sites (n), thermodynamic parameters, binding forces, Hill’s coefficient, and binding rate of gliclazide to protein calculated from two methods using the same equation were consistent at three different temperatures (298, 310, 318 K). This indicated that as a useful supplement to the conventional method, synchronous fluorescence spectroscopy could be used to study the mechanism of drugs and proteins. The conclusion was verified by UV/vis method. |
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Keywords: | Bovine serum albumin fluorescence spectroscopy gliclazide interaction synchronous fluorescence spectroscopy |
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