A Quantitative Determination of the Interaction of Pentamethylenetetrazole with the Sodium Ion by Sodium-23 NMR

The importance of the sodium ion transport in biological systems is well known. Numerous studies have dealt with compounds which affect ion transport across membrane systems. However, the mechanism of ion transport across the membrane is still not clear. Hence it is of interest to investigate the interaction of the sodium ion with biologically active compounds in a system where the complex membrane is absent. Such a method of investigation must be able to detect very weak interactions. Sodium-23 nuclear magnetic resonance fits well into this classification. This technique has been used by others¹ and by us² to investigate electrolyte solutions and biological or model systems. We have been interested in the chemistry of cyclopolymethy-lenetetrazoles for some time.³ These compounds are characterized by their strong stimulating action on the central nervous system and, in