聚丙烯微孔膜表面肝素化及其对低密度脂蛋白的吸附特性

为了赋予聚丙烯微孔膜(PPMM)选择性吸附低密度脂蛋白(LDL)的能力,发展了一种有效的PPMM表面共价固定肝素的方法.基于紫外引发丙烯酸的接枝聚合,通过碳二亚胺活化,以乙二胺为间隔臂,将肝素共价固定于PPMM表面,获得表面肝素化的PPMM.ATR-FTIR和XPS分析确证了修饰过程中膜表面基团及化学成分的变化.采用静...

SURFACE HEPARINIZATION OF POLYPROPYLENE MICROPOROUS MEMBRANES FOR SELECTIVE ADSORPTION OF LOW-DENSITY LIPOPROTEIN

To endow polypropylene microporous membrane(PPMM) with adsorption selectivity to low-density lipoprotein(LDL),a versatile method was developed to covalently immobilize heparin on the PPMM surface.This was achieved by grafting acrylic acid(AA) on the PPMM surface by UV irradiation,and subsequent chemical binding of heparin with ethylenediamine as a spacer.The membrane surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy.The hydrophilicity and blood compatibility of the PPMM surface were improved obviously by the surface heparinization.Water contact angle of the membrane surface was decreased from(138±7.2)° for the nascent PPMM to(35.1±2.7)° for the heparinized PPMM with 7.39 μg/cm2 heparin.An enzyme-linked immunosorbent assay(ELISA) was employed to analyze the adsorption of the LDL on the nascent and heparinized PPMMs.It was found that the surface heparinization greatly enhanced the adsorption of LDL both from single protein solution and binary protein solution containing human serum albumin(HSA),and the heparinized PPMM could selectively adsorb LDL from binary protein solution.In addition,the adsorbed LDL could be desorbed from the heparinized PPMM with sodium chloride solution,which indicated the adsorption was of reversibility.