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Multiple Paramagnetic Effects through a Tagged Reporter Protein |
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| Authors: | Aldo R Camacho‐Zarco Dr Francesca Munari Melanie Wegstroth Dr Wei‐Min Liu Prof Marcellus Ubbink Dr Stefan Becker Prof Markus Zweckstetter |
| Institution: | 1. Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 G?ttingen (Germany);2. German Center for Neurodegenerative Diseases (DZNE), G?ttingen (Germany);3. Center for the Molecular Physiology of the Brain, University Medical Center, G?ttingen (Germany);4. Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden (The Netherlands) |
| Abstract: | Paramagnetic effects provide unique information about the structure and dynamics of biomolecules. We developed a method in which the lanthanoid tag is not directly attached to the protein of interest, but instead to a “reporter” protein, which binds and then transmits paramagnetic information to the target. The designed method allows access to a large number of paramagnetic restraints and residual dipolar couplings produced from independent molecular alignments in high‐molecular‐weight proteins with unknown 3D structure |
| Keywords: | lanthanoids NMR spectroscopy paramagnetism proteins structural biology |