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Ultrafast Extraction of Proteins from Tissues Using Desorption by Impulsive Vibrational Excitation |
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| Authors: | Marcel Kwiatkowski Marcus Wurlitzer Maryam Omidi Ling Ren Sebastian Kruber Refat Nimer Dr. Wesley D. Robertson Priv.‐Doz. Dr. Andrea Horst Prof. Dr. R. J. Dwayne Miller Prof. Dr. Hartmut Schlüter |
| Affiliation: | 1. University Medical Center Hamburg‐Eppendorf, Department of Clinical Chemistry, Martinistrasse 52, Hamburg, 20246 (Germany);2. Max Planck Institute for the Structure and Dynamics of Matter, CFEL (Building 99), Luruper Chaussee 149, 22761 Hamburg (Germany) |
| Abstract: | A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL‐DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained. |
| Keywords: | mass spectrometry protein extraction proteomics picosecond IR laser tissue samples |