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NeuO: a Fluorescent Chemical Probe for Live Neuron Labeling |
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| Authors: | Jun Cheng Er Dr. Cheryl Leong Dr. Chai Lean Teoh Qiang Yuan Paolomi Merchant Matthew Dunn Prof. David Sulzer Prof. Dalibor Sames Dr. Akshay Bhinge Dongyoon Kim Seong‐Min Kim Prof. Myung‐Han Yoon Dr. Lawrence W. Stanton Prof. Shawn H. Je Dr. Seong‐Wook Yun Prof. Young‐Tae Chang |
| Affiliation: | 1. Department of Chemistry, National University of Singapore, 3 Science Drive 2, 117543 Singapore (Singapore);2. Singapore BioImaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, 138667 Singapore (Singapore);3. Duke‐NUS Graduate Medical School, National University of Singapore, 8 College Road, 169857 Singapore (Singapore);4. Department of Chemistry, Columbia University, 3000 Broadway MC3101, New York, NY 10027 (USA);5. Departments of Neurology and Psychiatry, Columbia University, 650 W 168th Street BB308, New York, NY 10032 (USA);6. Genome Institute of Singapore, Agency for Science, Technology and Research, 60 Biopolis Street, 138672 Singapore (Singapore);7. Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan‐gwagiro, Gwangju 500712 (Korea) |
| Abstract: | To address existing limitations in live neuron imaging, we have developed NeuO , a novel cell‐permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications. |
| Keywords: | BODIPY fluorescent probes imaging agents live neurons |