Determination of acetaldehyde by flow injection analysis with soluble or immobilized aldehyde dehydrogenase |
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| Authors: | Ala&#x;ddin M Almuaibed Alan Townshend |
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| Institution: | Department of Chemistry, University of Hull, Hull HU6 7RX Great Britain |
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| Abstract: | Acetaldehyde (0.18–7.7 × 10?4 M) in water is determined by using a double injection technique with the soluble enzyme or with a mini-column of aldehyde dehydrogenase immobilized on cyanogen-activated Sepharose 4B. The NADH produced is monitored spectrophotometrically. The sample throughput is ca. 40 h?1, and the immobilized enzyme is stable for at least a month. Ethanol up to 5% (v/v) does not interfere. |
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