Quantitation of enzymatically generated hydrogen peroxide based on aqueous peroxyoxalate chemiluminescence |
| |
| Authors: | M.L. Grayeski E.J. Woolf P.J. Helly |
| |
| Affiliation: | Chemistry Department, Seton Hall University, South Orange, NJ 07079 U.S.A. |
| |
| Abstract: | The method involves the reaction of 4,4′-{oxalyl bis[(trifluoromethylsulfonyl)imino]-ethylene}-bis(4-methylmorpholinium trifluoromethanesulfonate) with hydrogen peroxide in the presence of rhodamine-B. Precise measurements, with 1–3% relative standard deviation, can be made in both static and flow systems. In the flow system, the response to hydrogen peroxide is linear from 10?2 M hydrogen peroxide down to the limit of detection of 7 × 10?5 M. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
