Glucose enzyme electrode with extended linearity: Application to undiluted blood measurements |
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| Authors: | William H Mullen Fiona H Keedy Stephen J Churchouse Pankaj M Vadgama |
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| Institution: | Department of Physical Chemistry, The University, Newcastle upon Tyne NE1 7RU Great Britain;Department of Clinical Biochemistry and Metabolic Medicine, The Medical School, Framlington Place, Newcastle upon Tyne, NE2 4HH Great Britain |
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| Abstract: | An enzyme electrodes is described for glucose determination in unstirred, undiluted whole blood. The system comprises an H2O2-detecting electrode upon which is placed a membrane laminate incorporating glucose oxidase. The external membrane was pretreated with methyltrichlorosilane. The electrode response was linearly dependent on glucose concentration up to 50 mmol l?1 glucose, it had a decreased dependence on dissolved oxygen concentrations and gave response times of 30–90 s. Whole blood glucose measurements correlated well with a routine spectrophotometric method. |
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