A microsensor for adenosine-5′-triphosphate pH-sensitive field effect transistors |
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Authors: | Masao Gotoh Eiichi Tamiya Isao Karube Yasuo Kagawa |
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Affiliation: | Research Department, NOK Corporation, 4-3-1 Tsujidoshinmachi, Fujisawa 251 Japan;Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 227 Japan;Department of Biochemistry, Jichi Medical School, Minamikawachi, Tochigi-ken, 329-04 Japan |
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Abstract: | The sensor for adenosine-5′-triphosphate (ATP) is based on H+-ATPase immobilized via a polyvinylbutyral resin on a pH-sensitive field effect transistor. A linear relationship was obtained between the initial rate of change of the differential gate output voltage and the logarithm of the ATP concentration over the range 0.2–1.0 mM ATP. The optimum pH was 9.0 at 40°C but pH 7.0 was preferred for routine measurements. Only slight responses were obtained for 1 mM glucose, creatinine or urea. The ATP-sensing system exhibited a response to 1 mM ATP for at least 18 days. |
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