Spectrofluorimetric determination of 16α-hydroxyestrone by a reaction with hexacyanoferrate(III) and arginine in alkaline solution |
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| Authors: | Yoshihisa Yamaguchi Chozo Hayashi Kiyoshi Miyai |
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| Affiliation: | Central Laboratory for Clinical Investigation, Osaka University Hospital, 1-1-50 Fukushima, Osaka 553 Japan |
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| Abstract: | The reaction of 16α-hydroxyestrone with hexacyanoferrate(III) and arginine in alkaline solution at 95°C produces a fluorescent compound that is measured at 490 nm (λ(ex) = 395 nm). Most other steroids do not interfere. The calibration is linear for 0.5–50 μg of analyte. |
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