Determination of glycerol, 1,2-propanediol and triglycerides by high-performance liquid chromatography and a post-column reactor containing immobilized glycerol dehydrogenase |
| |
Authors: | Nobutoshi Kiba Kazuyuki Goto Motohisa Furusawa |
| |
Institution: | Department of Chemistry, Faculty of Engineering, Yamanashi University, Kofu 400 Japan |
| |
Abstract: | A fluorimetri method is described for the determination of glycerol, 1,2-propanediol and triglycerides in serum by high-performance liquid chromatography with an on-line post-column reactor containing immobilized glycerol dehydrogenase. Before separation, triglycerides are cleaved with lipase and esterase. The polyhydric alcohols are separated from each other on a Finepak SIL C18 (10 μm) column with water as eluent. The NADHI produced from the enzymatic reaction is monitored by fluorimetry. Calibration curves are linear between 0.01 mM and 1.0 mM for glycerol or 2.0 mM for 1,2-propanediol. The method gave satisfactory results for control sera. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|