Determination of glutathione in biological material by high-performance liquid chromatography with electrochemical detection |
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| Authors: | Wolfgang Buchberger Karl Winsauer |
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| Affiliation: | Department of Chemistry, Paracelsus Institute, A-4540 Bad Hall Austria;Institute of Chemistr, Analytical Department, University of Linz, A-4040 Linz Australia |
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| Abstract: | Glutathione in biological samples is extracted by perchloric acid and separated by ion-paier chromatography on a RP-18 phase. In a post-column reaction, glutathione is converted to an isoindole derivative by reaction with o-phthalaldehyde and detected at a galssy carbon electrode at 800 mV v. Ag/AgCl/3 M KCl. The detection limit is 40 pmol of glutathione injected. |
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