Room-temperature phosphorimetric determination of biogenic indole compounds adsorbed on a thin-layer chromatographic plate |
| |
Authors: | Naotaka Kuroda Hitoshi Nohta Yosuke Ohkura |
| |
Institution: | Faculty of Pharmaceutical Sciences, Kyushu University 62, Maidashi, Higashi-ku, Fukuoka 812 Japan |
| |
Abstract: | A simple and sensitive method is described for the determination of biogenic indole compounds adsorbed on cellulose or alumina plates for thin-layer chromatography by room-temperature phosphorimetry. The optimum conditions were investigated for 5-hydroxyindole-3-acetic acid and indole-3-acetic acid. The compounds are spotted on the plates, which are then sprayed successively with sodium citrate or sodium acetate, and sodiu iodide solutions. The plates are dried completely under a stream of dry nitrogen, and immediately dipped in molten paraffin. The phosphorescence is stable for at least 3 h even in moist air. The limits of detection for nine biogenic indole compounds tested are between 2 and 300 pmol per sample spot. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|