Mediated amperometric biosensors for d-galactose,glycolate and l-amino acids based on a ferrocene-modified carbon paste electrode |
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Authors: | Jonathan M. Dicks William J. Aston Graham Davis Anthony P.F. Turner |
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Affiliation: | Bioelectronics Division, Biotechnology Centre, Cranfield Institute of Technology, Cranfield, Bedfordshire, MK43 0AL Great Britain |
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Abstract: | Direct-current cyclic voltammetry is used to investigate the suitability of a ferrocene derivative as a mediator with galactose, glycolate and l-amino acid oxidases. The three enzymes coupled catalytically to ferrocene monocarboxylic acid exhibiting homogeneous second-order rate constants in the range 0.4 × 105 to 8.5 × 105 l mol?1 s?1. Enzyme electrodes which responded to d-galactose, glycolate or l-amino acids were constructed. The appropriate oxidase was retained behind a dialysis membrane at a carbon paste electrode containing the poorly soluble derivative 1,1′-dimethylferrocene. All the electrodes responded rapidly to millimolar concentrations of their respective substrates producing 95% of the steady-state current response in <2 min. This general method of biosensor construction should be widely applicable to oxidases and other oxidoreductase enzymes. |
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