Comparison of immobilized enzyme reactors for flow-injection systems |
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Authors: | Robert Q. Thompson Hidong Kim Charles E. Miller |
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Affiliation: | Chemistry Department, Oberlin College, Oberlin, OH 44074 U.S.A. |
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Abstract: | Straight, coiled, beaded, and packed-bed reactors containing immobilized glucose oxidase or l-(+)-lactate dehydrogenase were compared in terms of reaction rate, sensitivity, sample throughput, and sample dispersion. The enzymes were covalently attached to the inside walls of 5.0-cm long, 1.12-mm i.d. nylon tubes, and the resulting reactors were tested in a flow-injection system. The beaded enzymatically-active reactors (BEARs) were filled with solid glass beads of 0.5-mm or 1.0-mm diameter. Reactors with the larger beads had 2–4 times the activity, twice the sensitivity, and better throughput than the open reactors; they also minimized the physical and chemical contributions to dispersion. Packed-bed reactors were superior in the lactate determination, but the beaded reactors were better for the determination of glucose. With BEARs containing 1.0-mm beads, glucose was determined in the range 10–800 μM with a conversion efficiency of 0.056 mol of product per mole of substrate; for lactate, the range was 8–64 μM with a conversion efficiency of 0.13 mol mol?1. |
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