| 用吐温80—盐—水液—固萃取体系从兔肌中分离纯化乳酸脱氢酶 |
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| 引用本文： | 柳畅先 邓江天. 用吐温80—盐—水液—固萃取体系从兔肌中分离纯化乳酸脱氢酶[J]. 化学研究与应用, 1997, 9(5): 493-496 |
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| 作者姓名： | 柳畅先 邓江天 |
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| 作者单位： | 中南民族学院化学系 |
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| 摘 要： | 用吐温８０－盐－水液－固萃取体系从兔肌中分离纯化乳酸脱氢酶＊柳畅先邓江天雷锦桂游文淑李步海（中南民族学院化学系武汉４３００７４）关键词液－固萃取吐温８０乳酸脱氢酶中图分类号Ｏ６５８．２非有机溶剂液－固萃取体系是一种新的萃取体系，其特点之一是聚合物固相...
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| 关 键 词： | 萃取 吐温80 乳酸 脱氢酶 免肌 |
SEPARATION AND PURIFICATION OF LACTATE DEHYDROGENASE FROM RABBIT MUSCLE BY USING THE LIQUID SOLID EXTRACTION SYSTEM OF TWEEN80 (NH 4) 2SO 4 H 2O |
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| Liu Changxian,Deng Jiangtian,Lei Jingui,You Wenshu and Li Buhai. SEPARATION AND PURIFICATION OF LACTATE DEHYDROGENASE FROM RABBIT MUSCLE BY USING THE LIQUID SOLID EXTRACTION SYSTEM OF TWEEN80 (NH 4) 2SO 4 H 2O[J]. Chemical Research and Application, 1997, 9(5): 493-496 |
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| Authors: | Liu Changxian Deng Jiangtian Lei Jingui You Wenshu Li Buhai |
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| Abstract: | The distribution behaviour of lactate dehydrogenase and bovine serum albumin in the liquid solid extraction system of Tween80 (NH 4) 2SO 4 H 2O was investigated.The effect of pH and (NH 4) 2SO 4 concentration on the E of the enzyme and the protein was given in detail.The experimental results indicate that in the specified condition,lactate dehydrogenase remains in the aqueous phase and a large fraction of other proteins were extracted into the solid phase.This system was applied to the separation and purification of lactate dehydrogenase from rabbit muscle.The purification fold is 1.8,and recovery is 80%. |
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| Keywords: | Liquid solid extraction Tween80 Lactate dehydrogenase |
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