Bioorthogonal oxime ligation mediated in vivo cancer targeting
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| Authors: | Li Tang Qian Yin Yunxiang Xu Qin Zhou Kaimin Cai Jonathan Yen Lawrence W Dobrucki Jianjun Cheng |
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| Institution: | a Department of Materials Science and Engineering , University of Illinois at Urbana–Champaign , 1304 West Green Street , Urbana , IL 61801 , USA . Email: ; Fax: +1-217-333-2736 ; Tel: +1-217-244-3924 ; b Department of Pharmaceutical Science , Guangdong Pharmaceutical University , Guangzhou , Guangdong 510006 , China ; c Department of Bioengineering , University of Illinois at Urbana–Champaign , Urbana , IL 61801 , USA |
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| Abstract: | Current cancer targeting relying on specific biological interaction between the cell surface antigen and respective antibody or its analogue has proven to be effective in the treatment of different cancers; however, this strategy has its own limitations, such as the heterogeneity of cancer cells and immunogenicity of the biomacromolecule binding ligands. Bioorthogonal chemical conjugation has emerged as an attractive alternative to biological interaction for in vivo cancer targeting. Here, we report an in vivo cancer targeting strategy mediated by bioorthogonal oxime ligation. An oxyamine group, the artificial target, is introduced onto 4T1 murine breast cancer cells through liposome delivery and fusion. Poly(ethylene glycol)-polylactide (PEG-PLA) nanoparticles (NPs) are surface-functionalized with aldehyde groups as targeting ligands. The improved in vivo cancer targeting of PEG-PLA NPs is achieved through specific and efficient chemical reaction between the oxyamine and aldehyde groups. |
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