亚硝酸异丁酯遗传毒性效应的研究

摘要:探讨亚硝酸异丁酯致突变作用,根据《化学品毒性鉴定技术规范》 ,采用鼠伤寒沙门氏菌回复突变实验( Ames实验)和小鼠骨髓嗜多染红细胞( PCE)微核实验,进行亚硝酸异丁酯的遗传毒性研究。 Ames 实验使用的菌株为鼠 伤寒沙门氏菌( Salmonella typhimurium) 组氨酸缺陷型( his-) 菌株 TA97a、TA98、TA100 及 TA102,实验共设 10 个剂 量组,剂量区间在 250 ~ 5 000 μg / 皿,同时设阴性对照组和阳性对照组,采用平板掺入法,观察每皿回变菌落数;微核实验设 11 mg / kg、41 mg / kg 及 103 mg / kg 三个剂量组,设阴性对照组和阳性对照组,检测骨髓嗜多染红细胞微核率。 结果提示亚硝酸异丁酯在 Ames 实验中未呈现致突变作用,但在微核实验中 103 mg / kg 时对小鼠有致突变作用。

Study on the Genotoxic Toxicity of Isobutyl Nitr

Abstract:To explore the mutagenic effects of isobutyl nitrite, according to the “ Technical Specifications for theIdentification of Chemical Toxicity” , Salmonella typhimurium reverse mutation test ( Ames test) and mouse bone marrow polychromatic erythrocyte micronucleus test are used for the genotoxicity of isobutyl nitrite. The strains used in the Ames experiment are the histidine-deficient strains of S. typhimurium TA97a, TA98, TA100 and TA102. There were 10 dose groups in the experiment, and the dose range is from 250 to 5 000 μg / dish, setting up negative and positive control groups at the same time. Adopting plate incorporation method to observe the number of colonies per dish. The micronucleus test has three dose groups of 11 mg / kg, 41 mg / kg and 103 mg / kg. A negative control group and a positive control group are also set up to detect micronucleus rate of bone marrow polychromatic erythrocytes. The result indicate that isobutyl nitrite did not show mutagenic effects in the Ames experiment, and inthe micronucleus experiment, there was mutagenic effect on mice at 103 mg / kg.