Isolation and purification of ginkgo flavonol glycosides from Ginkgo biloba leaves by high-speed counter-current chromatography |
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Authors: | Zhang Qiang Chen Li-Juan Ye Hao-Yu Gao Lei Hou Wenli Tang Minghai Yang Guangli Zhong Zhenhua Yuan Yuan Peng Aihua |
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Institution: | Institute for Chemical Engineering, Sichuan University, Chengdu, China. |
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Abstract: | A high-speed counter-current chromatography method was developed for the separation and purification of bioactive flavonol glycosides from a crude ethanol extract of Ginkgo biloba leaves. The separation was performed with a two-phase solvent system composed of n-hexane-butanol-ethyl acetate-methanol-0.5% acetic acid (1:0.5:3.5:1:4, v/v) and three pure compounds were eluted in high purities in a one-step separation. Their purities were determined by HPLC and identified by MS,(1)H-NMR, and(13)C-NMR. |
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Keywords: | Flavonol glycosides Ginkgo biloba leaves High‐speed counter‐current chromatography |
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