NRPS substrate promiscuity diversifies the xenematides |
| |
Authors: | Crawford Jason M Portmann Cyril Kontnik Renee Walsh Christopher T Clardy Jon |
| |
Institution: | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA. |
| |
Abstract: | Xenematide, a cyclic depsipeptide antibiotic produced by Xenorhabdus nematophila, had a candidate nonribosomal peptide synthetase (NRPS) with atypical features. Differential metabolite analysis between a mutant and wildtype validated that this stand-alone NRPS was required for xenematide production, and further analysis led to a series of new xenematide derivatives encoded by the same NRPS. Our results indicate that adenylation domain promiscuity and relaxed downstream processing in the X. nematophila NRPS provide a conduit for xenematide diversification. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|