Laserspray and Matrix-Assisted Ionization Inlet Coupled to High-Field FT-ICR Mass Spectrometry for Peptide and Protein Analysis |
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Authors: | Leonard Nyadong Ellen D Inutan Xu Wang Christopher L Hendrickson Sarah Trimpin Alan G Marshall |
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Institution: | 1. National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, 32310, USA 2. Department of Chemistry, Wayne State University, Detroit, MI, 48202, USA 3. Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, 32303, USA
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Abstract: | We present the first coupling of laser spray ionization inlet (LSII) and matrix assisted ionization inlet (MAII) to high-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) for generation of electrospray-like ions to take advantage of increased sensitivity, mass range, and mass resolving power afforded by multiple charging. We apply the technique to top-down protein analysis and characterization of metalloproteins. We also present a novel method for generation of multiply-charged copper–peptide complexes with varying degrees of copper adduction by LSII. We show an application of the generated copper–peptide complexes for protein charge state and molecular weight determination, particularly useful for an instrument such as a linear ion trap mass analyzer. ![></img> </span> </span></td>
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