| 一种含芴基的钌(II)配合物的合成及DNA键合性质 |
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| 引用本文： | 姚威,吴宝燕,高丽华,王科志.一种含芴基的钌(II)配合物的合成及DNA键合性质[J].物理化学学报,2007,23(2):237-241. |
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| 作者姓名： | 姚威 吴宝燕 高丽华 王科志 |
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| 作者单位： | Department of Chemistry, Beijing Normal University, Beijing 100875, P. R. China;2.School of Chemical and Environment Engineering, Beijing Technology and Business University, Beijing 100037, P. R. China |
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| 基金项目： | 国家自然科学基金;北京市自然科学基金;高等学校博士学科点专项科研项目 |
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| 摘 要： | 合成了一种新的钌(II)配合物Ru(bpy)2(Hfip)](ClO4)2, 其中bpy代表2,2′-联吡啶, Hfip代表2-(9H-芴-2-基)-1H-咪唑-4,5-f]-1,10]-邻菲啰啉. 通过紫外可见光谱、荧光光谱、稳态荧光淬灭、与溴化乙锭的竞争实验、粘度测量和DNA热变性研究了该配合物与小牛胸腺DNA的键合性质. 结果表明, 该配合物能嵌入键合DNA, 键合常数Kb=8.6×105 L·mol-1 (50 mmol·L-1 NaCl).
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| 关 键 词： | 钌(II) DNA 邻菲啰啉 紫外可见光谱 荧光光谱 |
| 收稿时间： | 2006-08-07 |
| 修稿时间： | 2006-08-07 |
DNA-binding Properties of a Fluorenyl-Containing Ru(Ⅱ) Complex |
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| YAO Wei,WU Bao-Yan,GAO Li-Hua,WANG Ke-Zhi.DNA-binding Properties of a Fluorenyl-Containing Ru(Ⅱ) Complex[J].Acta Physico-Chimica Sinica,2007,23(2):237-241. |
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| Authors: | YAO Wei WU Bao-Yan GAO Li-Hua WANG Ke-Zhi |
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| Institution: | Department of Chemistry, Beijing Normal University, Beijing 100875, P. R. China;2.School of Chemical and Environment Engineering, Beijing Technology and Business University, Beijing 100037, P. R. China |
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| Abstract: | A ruthenium(II) complex of Ru(bpy)2(Hfip)](ClO4)2 (bpy=2,2′-bipyridine, Hfip=2-(9H-fluoren-2-yl)-1H-imidazolo-4,5-f]-1,10]-phenanthrolin) was synthesized. The DNA binding properties of the complex were studied by means of electronic absorption and emission spectroscopic titrations, steady-state emission quenching by Fe(CN)4-6, viscosity measurements, DNA competitive binding with ethidium bromide, and thermal denaturation of DNA. The results indicated that the complex bound to calf-thymus DNA in an intercalative mode with a binding constant of 8.6×105 L·mol-1 in the presence of 50 mmol·L-1 NaCl solution. |
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| Keywords: | DNA |
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