Rapid determination of corticosteroids in pharmaceuticals by flow injection analysis |
| |
Authors: | John B Landis |
| |
Institution: | The Upjohn Company, Kalamazoo Michigan 49001 U.S.A. |
| |
Abstract: | The proposed method is based on the reduction of blue tetrazolium by the steroid in an alkaline medium to form a highly colored formazan. The effects of reagent concentration, temperature, flow rate, and manifold design on the reaction are discussed for a typical steroid, methylprednisolone acetate. Analytical readout is obtained within 30 s after sample introduction and up to 100 samples/h can be processed with baseline resolution between peaks. Typical relative standard deviations of 0.5% are obtained with 10-μ1 injection volumes. Results obtained by flow injection analysis are similar to those obtained with the AutoAnalyzer technique. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|