Factors affecting the capillary electrophoresis of ricin, a toxic glycoprotein |
| |
Authors: | Harry B. Hines and Ernst E. Brueggemann |
| |
Affiliation: | Toxinology Division, United States Army Medical Research Institute For Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA |
| |
Abstract: | Conditions for the analysis of ricin with capillary electrophoresis were investigated. Uncoated and coated columns were tested with a variety of different buffer combinations which included different principal components, pH, ionic strength, and additives. Of the combinations tested, uncoated columns used with either zwitterionic salts or putrescine gave the best results. Multiple peaks were resolved with these conditions. Coated columns generally yielded between 1000 and 5000 plates with several buffer combinations. Ricin may be analyzed faster and with greater resolution with capillary electrophoresis employing untreated fused-silica columns than by using other chromatographic techniques. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|