Protein separation on a polar-copolymerized C8 stationary phase

A novel polar-copolymerized C₈ stationary phase composed of octyl and chloropropyl (C₈-C₃Cl)-bonded silica has been developed for protein separation. Separation of standard proteins on this homemade column was investigated and compared with separation on a Waters Symmetry 300 C₄ column. Results indicated that the chromatographic performance of the homemade column in the separation of proteins was excellent. The new polar copolymerized C₈ stationary phase enabled glycoprotein separation with good resolution and reproducibility. It was also used for purification of ovalbumin glycoprotein in order to improve identification of the protein.