Optical anisotropy of a biological tissue under conditions of immersion clearing and without them |
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| Authors: | A V Papaev G V Simonenko V V Tuchin T P Denisova |
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| Institution: | (1) Research and Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russia;(2) Saratov State Medical University, Saratov, 410012, Russia |
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| Abstract: | The optical anisotropy of a biological tissue is studied under conditions of immersion clearing of the tissue and without them. It is shown that the rate of immersion clearing of a biological tissue depends on its type and is determined by the rate of diffusion of immersion liquids into the sample. The parameters that characterize the rate of diffusion of an immersion liquid into a biological tissue are determined. The types of anisotropy of different samples of biological tissues are found. Based on the Müller matrix formalism, a model for calculating the spectrum of collimated polarized transmission of a biological tissue is constructed. Comparison of calculated and experimental transmission spectra of biological tissues showed their good quantitative coincidence. |
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