Interactions between dextran-modified poly(methyl methacrylate) latex particles and concanavalin A |
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Authors: | C-S Chern C-K Lee Y-J Tsai |
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Institution: | (1) Department of Chemical Engineering National Taiwan University of Science and Technology, 43 Keelung Rd. Sec. 4, Taipei 106, Taiwan e-mail: chern@ch.ntust.edu.tw, TW |
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Abstract: | The electrostatic and affinity interactions between concanavalin A (Con A) and dextran-modified latex particles were investigated.
The ratio of the initial number of dextran active sites to that of Con A molecules plays an important role in determining
the structure of flocs formed during adsorption and, thereby, has an influence on the amount of adsorbed Con A and the subsequent
elution yield. NaCl and d-glucose, used successively in the two-step desorption of Con A, were employed to study the relative importance of electrostatic
and affinity interactions involved in the Con A adsorption process. A significant fraction of adsorbed Con A is attributed
to the electrostatic interaction mechanism. The feasibility of using the dextran-modified latex products to purify Con A from
Jack bean meal was demonstrated.
Received: 22 February 2000 Accepted: 19 September 2000 |
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Keywords: | Affinity/electrostatic interactions Dextran-modified latex particles Concanavalin A (Con A) Adsorption/desorption |
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