Organophosphorus insecticides extraction and heterogeneous oxidation on column for analysis with an acetylcholinesterase (AChE) biosensor |
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Authors: | Dondoi Madalina Petruta Bucur Bogdan Danet Andrei Florin Toader Constantin Nicolae Barthelmebs Lise Marty Jean-Louis |
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Affiliation: | a University of Bucharest, Faculty of Chemistry, Sos. Panduri, No. 90-92, 050657 Bucharest, Romania b Université de Perpignan, BIOMEM, 52, Av. Paul Alduy, 66860 Perpignan Cedex, France c Scientifical Research Center for Defence NBC and Ecology, Sos. Oltenitei, No. 225, Sector 4, Bucharest, Romania |
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Abstract: | This paper presents an analysis method for organophosphorus insecticides based on AChE biosensors coupled with a preconcentration and oxidation on a solid phase column. Three organic solvents, acetonitrile (ACN), ethanol and methanol were tested for their influence on AChE activity, insecticide inhibition and their ability to elute the adsorbed insecticides. Our results showed that ACN in a concentration of 5% (v/v) had the less negative effect on biosensor analysis and was the most appropriate organic solvent for the column elution. The presence of the organic solvent in the incubation media of the biosensor was found to induce a reduction of the inhibition percentages. The inhibition of the biosensors was performed in phosphate buffer with 5% (v/v) ACN, while the initial and remaining response of the biosensors were measured in PBS. In these conditions, the LODs of paraoxon and dichlorvos were measured with or without a preconcentration step. The LODs of the AChE biosensor without sample preconcentration were 8 × 10−8 M for paraoxon and 1 × 10−7 M dichlorvos and the LOD obtained after the preconcentration step were 2.5 × 10−8 M for paraoxon and 2.5 × 10−8 M for dichlorvos. Moreover, the use of the column allowed the heterogeneous oxidation of organophosphorus insecticides for improved LOD. |
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Keywords: | Acetylcholinesterase biosensor Insecticide analysis Column extraction Organic phase biosensors Screen-printed electrode |
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