Nanoporous niobium oxide for label-free detection of DNA hybridization events |
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Authors: | Choi Jinsub Lim Jae Hoon Rho Sangchul Jahng Deokjin Lee Jaeyoung Kim Kyung Ja |
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Affiliation: | Nanomaterials Application Division, Korea Institute of Ceramic Engineering and Technology (KICET), Seoul 153-801, South Korea. jinsub@kicet.re.kr |
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Abstract: | We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1nM. |
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