Quantitative determination of glyphosate in human serum by 1H NMR spectroscopy |
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Authors: | Cartigny Bernard Azaroual Nathalie Imbenotte Michel Mathieu Daniel Parmentier Erika Vermeersch Gaston Lhermitte Michel |
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Institution: | Laboratoire de Toxicologie et Génopathies, H?pital Calmette, 59045 Lille Cedex, France. |
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Abstract: | The determination and quantification of glyphosate in serum using (1)H NMR spectroscopy is reported. This method permitted serum samples to be analysed without derivatization or any other sample pre-treatment, using 3-trimethylsilyl 2,2',3,3'-tetradeuteropropionic acid (TSP-d(4)) as a qualitative and quantitative standard. Characterization of the herbicide N-(phosphonomethyl)glycine was performed by analysing chemical shifts and coupling constant patterns. Quantification was performed by relative integration of CH(2)-P protons to the TSP-d(4) resonance peak. The method was tested for repeatability (n=5) and yielded coefficients of variation of 1% and 3%, respectively: detection and quantification limits were also determined and were 0.03 and 0.1mmol/L, respectively. The method was applied to the quantification of glyphosate in a case of acute poisoning. |
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