Temperature-dependent separation of bryostatin 18 and 10 by high performance liquid chromatography |
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| Authors: | F Lu G J Yang L L Liu X F Shen H W Lin Y H Yi |
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| Institution: | (1) School of Pharmacy, Second Military Medical University, GuoHe Road 101, 200433 Shanghai, China;(2) Shanghai Institute of Pharmaceutical Industry, North Zhongshan Road 1111, 20043, Shanghai, China |
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| Abstract: | Summary The temperature-dependent separation of bryostatins by HPLC was examined on an octadecyl bonded stationary phase, using column
temperatures between 0 and 40°C and mobile phase temperatures from 0 to 25°C. The retention time and resolution of bryostatins
changed drastically and separation improved with decreasing temperature. A column temperature of less than 5°C and a mobile
phase temperature of less than 15°C is recommended for a good resolution of bryostatins for routine work. |
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| Keywords: | Column liquid chromatography Temperature-dependent separations Bryostatins |
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