miR-370-3p Regulates Adipogenesis through Targeting Mknk1 |
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| Authors: | Peiwen Zhang Xinrong Li Shunhua Zhang Shuang Wu Qian Xiao Yang Gu Xinyu Guo Xutao Lin Lei Chen Ye Zhao Lili Niu Guoqing Tang Yanzhi Jiang Linyuan Shen Li Zhu |
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| Institution: | 1.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; (P.Z.); (X.L.); (S.Z.); (S.W.); (Q.X.); (Y.G.); (X.G.); (X.L.); (L.C.); (Y.Z.); (L.N.); (G.T.); (Y.J.);2.Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China |
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| Abstract: | Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat accumulation in the body. We found that miR-370-3p was expressed at lower levels in the fat mass of mice on a high-fat diet than in mice on a normal control diet. Furthermore, our data showed that the overexpression of miR-370-3p significantly suppressed the mRNA expression levels of adipogenic markers. Thus, miR-370-3p overexpression reduced lipid accumulation. Conversely, the inhibition of miR-370-3p suppressed 3T3-L1 preadipocyte proliferation and promoted preadipocyte differentiation. In addition, Mknk1, a target gene of miR-370-3p, plays an opposing role in preadipocyte proliferation and differentiation. Moreover, consistent results from in vitro as well as in vivo experiments suggest that the inhibition of fat accumulation by miR-370-3p may result from the inhibition of saturated fatty acids that promote the accumulation of polyunsaturated fatty acids. In conclusion, these results suggest that miR-370-3p plays an important role in adipogenesis and fatty acid metabolism through the regulation of Mknk1. |
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| Keywords: | miR-370-3p adipogenesis proliferation differentiation fatty acid composition |
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