Efficient Production of a Thermophilic 2-Deoxyribose-5-Phosphate Aldolase in Glucose-Limited Fed-Batch Cultivations of <Emphasis Type="Italic">Escherichia coli</Emphasis> by Continuous Lactose Induction Strategy |
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Authors: | Xiao-Lin Pei Qiu-Yan Wang Cheng-Lu Li Xiao-Feng Qiu Kai-Lin Xie Li-Feng Huang An-Ming Wang Zhao-Wu Zeng Tian Xie |
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Institution: | (1) Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou, 310012, People’s Republic of China; |
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Abstract: | The production of a thermophilic 2-deoxyribose-5-phosphate aldolases (DERA) in Escherichia coli BL21 under continuous lactose induction strategy was investigated. The process was combined with the exponential feeding
method, controlling the feeding rate to maintain the specific growth rate at 0.15 h−1. The results indicate that the lactose concentration in the feed medium affected directly the expression of the target protein.
The use of 50 g/L in the feed medium resulted in the biomass concentration of 39.3 g DCW/L, and an expression level of above
30%, and the maximum final DERA concentration of 16,200 U/L. Furthermore, the acetate concentration remained at a low level
in the fed-batch phase, less than 0.5 g/L. In conclusion, combining glucose feeding with lactose induction is a more powerful
way to achieve high cell density cultures and to efficiently produce the thermophilic DERA. The results also indicate the
potential industrial utility in the scale production of other recombinant proteins. |
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