| TaqMan MGB实时荧光PCR对转基因大豆定量检测的研究 |
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| 引用本文： | 黄东东,翁少萍,吕玲,常迪,何建国.TaqMan MGB实时荧光PCR对转基因大豆定量检测的研究[J].中山大学学报(自然科学版),2008,47(3):140-142. |
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| 作者姓名： | 黄东东 翁少萍 吕玲 常迪 何建国 |
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| 作者单位： | 中山大学生命科学学院,广东,广州,510275 |
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| 摘 要： | 利用实时荧光定量PCR技术，根据转基因大豆（Roundup ReadyTM）的外源基因35S启动子序列设计引物和TaqMan MGB探针，对大豆粉中Roundup ReadyTM大豆含量进行了定量检测，根据这个检测体系建立了35S启动子Ct值与样品中转基因成分数量之间的标准曲线和线性回归方程(相关系数r2: 0.9942)。本研究设计的方法还可以应用到多组分的食品、饲料等加工产品，检测转基因成分的含量，并可作为转基因食品常规PCR定性检测方法。
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| 关 键 词： | 转基因大豆 实时定量PCR TaqMan MGB探针 基因 生物技术 食品技术 |
| 收稿时间： | 2007-10-19; |
Detection of Genetically Modified Soybean by TaqMan MGB Real-time PCR |
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| HUANG Dong-dong,WENG Shao-ping,LV Ling,CHANG Di,HE Jian-guo.Detection of Genetically Modified Soybean by TaqMan MGB Real-time PCR[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2008,47(3):140-142. |
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| Authors: | HUANG Dong-dong WENG Shao-ping LV Ling CHANG Di HE Jian-guo |
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| Institution: | (School of Life Sciences,Sun Yat sen University,Guangzhou 510275, China) |
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| Abstract: | According to the PCR primers and TaqMan MGB probe of exogenous 35S promotor, the quantitative detection of genetically modified soybean ( Roundup ReadyTM) was established by real time PCR technology. According to this detection system, the standard curve of Ct vs genetically modified organism quantity was generated and a linear regression equation was obtained (r2 : 0.9942). The results demonstrated that this method could be used in quantificational detection of multiple organism food. |
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| Keywords: | genetically modified soybean real time quantitative PCR TaqMan MGB probe gene biotechnolgy food technology |
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