Photoregulation of RNA digestion by RNase H with azobenzene-tethered DNA |
| |
Authors: | Matsunaga Daijiro Asanuma Hiroyuki Komiyama Makoto |
| |
Affiliation: | Research Center for Advance Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. |
| |
Abstract: | RNA digestion by RNase H, which is responsible for the antisense effect, was efficiently photoregulated by use of the duplex of azobenzene-tethered sense DNA and native antisense DNA. In the dark, RNA digestion was suppressed because antisense DNA was strongly hybridized with azobenzene-tethered sense DNA, and accordingly RNA was isolated. On UV irradiation, antisense DNA was released from the azobenzene-tethered DNA due to the trans-to-cis isomerization and hybridized with RNA, which was digested by RNase H. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|