Determination of nitrite, nitrate, bromide, and iodide in seawater by ion chromatography with UV detection using dilauryldimethylammonium-coated monolithic ODS columns and sodium chloride as an eluent期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Determination of nitrite, nitrate, bromide, and iodide in seawater by ion chromatography with UV detection using dilauryldimethylammonium-coated monolithic ODS columns and sodium chloride as an eluent

Authors:

Kazuaki Ito Ryosuke Nomura Takuya Fujii Masahito Tanaka Tomoaki Tsumura Hiroyuki Shibata Takeshi Hirokawa

Institution:

1. Department of Biotechnology and Chemistry, Faulty of Engineering, Kinki University, 1 Umenobe, Takaya, Higashi-Hiroshima, 739-2116, Japan
2. Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739-8527, Japan

Abstract:

A method was developed for determination of inorganic anions, including nitrite (NO ₂ ^? ), nitrate (NO ₃ ^? ), bromide (Br^?), and iodide (I^?), in seawater by ion chromatography (IC). The IC system used two dilauryldimethylammonium bromide (DDAB)-coated monolithic ODS columns (50?×?4.6?mm i.d. and 100?×?4.6?mm i.d.) connected in series for separation of the ions. Aqueous NaCl (0.5?mol/L; flow rate, 3?mL/min) containing 5?mmol/L phosphate buffer (pH 5) was used as the eluent, and detection was with a UV detector at 225?nm. The monolithic ODS columns were coated and equilibrated with a 1-mmol/L DDAB solution (in H₂O/methanol, 90:10 v/v). The hydrophilic ions (NO ₂ ^? , NO ₃ ^? , and Br^?) were separated within 3?min and the retention time of I^? was 16?min. No interferences from matrix ions, such as chloride and sulfate ions, were observed in 35?‰ artificial seawater. The detection limits were 0.6?μg/L for NO ₂ ^? , 1.1?μg/L for NO ₃ ^? , 70?μg/L for Br^?, and 1.6?μg/L for I^? with a 200-μL sample injection. The performance of the coated columns was maintained without addition of DDAB in the eluent. The IC system was successfully applied to real seawater samples with recovery rates of 94–108?% for all ions.

Figure
The hydrophilic ions (NO ₂ ^? , NO ₃ ^? , and Br^?) and I^? in seawater was determined by a single run using the IC system consisting of two dilauryldimethylammonium bromide (DDAB)-coated monolithic ODS columns (50?×?4.6?mm i.d. and 100?×?4.6?mm i.d.) connected in series, NaCl (0.5?mol/L; flow rate, 3?mL/min) containing 5?mmol/L phosphate buffer (pH 5) as the eluent, and a UV detector (225?nm). No interferences from matrix ions, such as chloride and sulfate ions, were observed in 35?‰ artificial and real seawaters.

Keywords:

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