| 2-氨基-5-硝基吡啶在基体辅助激光解吸/电离质谱法测定核酸分子中的应用 |
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| 引用本文： | 黎军,邓慧敏,赖志辉,赵善楷.2-氨基-5-硝基吡啶在基体辅助激光解吸/电离质谱法测定核酸分子中的应用[J].分析化学,1999,27(8):917-919. |
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| 作者姓名： | 黎军 邓慧敏 赖志辉 赵善楷 |
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| 作者单位： | 中山大学测试中心,广州,510275 |
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| 基金项目： | 国家自然科学基金(No.29170244)资助课题 |
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| 摘 要： | 报道以2-氨基-5-硝基吡啶为基体用基体辅助激光解吸/电离质谱法(MALDI-MS)对DNA样品进行测定并研究它们的激光质谱特征,结果表明,2-氨基-5-硝基吡啶是DNA的一高效基体.用柠檬酸铵和NH_4~+阳离子交换树脂除去试剂和样品中的碱金属离子,能显著地提高解吸/电离DNA分子的效率.
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| 关 键 词： | 基体辅助激光解吸/电离质谱法 核酸 2-氨基-5-硝基吡啶 |
| 修稿时间： | 1998年9月21日 |
The Usage of 2-Amino-5-nitropyridine in the Measurement of Deoxyribonucleic Acid by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry |
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| Li Jun,Deng Huimin,Lai Zhihui,Zhao Shankai.The Usage of 2-Amino-5-nitropyridine in the Measurement of Deoxyribonucleic Acid by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,1999,27(8):917-919. |
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| Authors: | Li Jun Deng Huimin Lai Zhihui Zhao Shankai |
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| Abstract: | This paper reports the study on matrix-assisted laser desorpdon/ionization mass spectrometry (MALDI-MS) of deoxyribonucleic acid (DNA) oligomers, using matrix 2-amino-5-nitropyridine. The DNA characters in MALDI-MS are discussed. The results shows that 2-amino-S-nitropyridine has high efficiency in desorbing and ionizing DNA molecules. Using ammonium citrate and NH4+ ion exchange resin to eliminate alkali metal ions, the effect of desorbing and ionizing DNA has been improved distinctly. |
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| Keywords: | Matriix-assisted laser desorptian/ionization mass spectrometry nucleodde 2-amino-5-nitropyridine |
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