| 高效液相色谱-圆二色检测法分析甲霜灵的对映体纯度 |
| |
| 引用本文： | 陈胜文,林坤德,席萌,刘维屏.高效液相色谱-圆二色检测法分析甲霜灵的对映体纯度[J].分析化学,2006,34(4):525-528. |
| |
| 作者姓名： | 陈胜文 林坤德 席萌 刘维屏 |
| |
| 作者单位： | 浙江大学环境科学研究所,杭州,310029;浙江工业大学环境科学研究中心,杭州,310032 |
| |
| 基金项目： | 中国科学院资助项目;国家重点基础研究发展计划(973计划) |
| |
| 摘 要： | 以手性农药甲霜灵为研究对象,使用非手性高效液相色谱在不拆分对映体的条件下,利用圆二色检测器所测的各向异性系数(g)测定手性对映体纯度。实验结果表明,g与对映体过剩率(ee)具有良好的线性关系;通过比较g所测ee与手性色谱所测的ee,二者所测ee相对平均偏差小于3.0%,说明该方法具有较高的准确性,可应用于手性化合物对映体纯度的测定。
|
| 关 键 词： | 圆二色检测器 高效液相色谱 对映体纯度 各向异性系数 甲霜灵 |
| 收稿时间： | 06 1 2005 12:00AM |
| 修稿时间： | 2005-06-012005-09-22 |
Determination of Metalaxyl Enantiomeric Purity by Nonchiral High Performance Liquid Chromatography with Circular Dichroism Detector |
| |
| Chen Shengwen,Lin Kunde,Xi Meng,Liu Weiping.Determination of Metalaxyl Enantiomeric Purity by Nonchiral High Performance Liquid Chromatography with Circular Dichroism Detector[J].Chinese Journal of Analytical Chemistry,2006,34(4):525-528. |
| |
| Authors: | Chen Shengwen Lin Kunde Xi Meng Liu Weiping |
| |
| Institution: | 1. Institute of Environmental Science, Zhejiang University, Hangzhou 310029;2 . Research Center of Environmental Science, Zhejiang University of Technology, Hangzhou 310032 |
| |
| Abstract: | A novel method for the determination of enantiomeric purity of chiral compounds by nonchiral high performance liquid chromatography(HPLC) coupled with circular dichroism(CD) detector was developed.The principle of the method was proposed and illustrated by the analysis of enantiomeric purity of metalaxyl.The results indicated that g values(anisotropy factor) had a good linearity with enantiomeric excess(ee) and correlation coefficient was 0.9974.The relative average deviations between the ee evaluated by the developed method and those determined by chiral HPLC were less than 3.0%.The proposed method has a high accuracy and shows a good perspective to determine the ee of chiral compounds. |
| |
| Keywords: | Circular dichroism high performance liquid chromatography enantiomericpurity anisotropy(factor) metalaxyl |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
