|
|||||
|
|
Template-directed photoreversible ligation of DNA via 7-carboxyvinyl-7-deaza-2′-deoxyadenosine |
|
| Authors: | Isao Saito Yohei Miyauchi Kenzo Fujimoto |
| Institution: | a NEWCAT Institute, Graduate School of Engineering, Nihon University and SORST, Japan Science and Technology Agency, Tamura, Koriyama 963-8642, Japan b Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan c Japan Advanced Institute of Science and Technology, Tatsunokuchi, Nomi 923-1293, Japan |
| Abstract: | An efficient template-directed photoligation of oligodeoxynucleotide (ODN) using 7-deaza-2′-deoxyadenosine derivative VZA is described. When ODN containing VZA at the 5′ end was photoirradiated with ODNs containing a pyrimidine base at the 3′ end in the presence of template ODN, rapid and efficient ligation (cycloaddition reaction) was observed without any byproduct formation. ODNs containing VZA showed an extremely high reactivity as compared with those reported in previous photoligations. |
| Keywords: | DNA Photoligation Deazaadenosine |
| 本文献已被 ScienceDirect 等数据库收录！ | |