| 毛细管电泳在生物大分子分析中的应用研究进展 |
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| 引用本文： | 彭乐,程佳伟,李淑楠,李文龙. 毛细管电泳在生物大分子分析中的应用研究进展[J]. 分析测试技术与仪器, 2022, 28(4): 384-391. DOI: 10.16495/j.1006-3757.2022.04.003 |
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| 作者姓名： | 彭乐 程佳伟 李淑楠 李文龙 |
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| 作者单位： | 1.天津中医药大学 中药制药工程学院，天津 300193 |
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| 基金项目： | 武汉市科技计划项目（2018060402011258），天津市科技计划项目（20ZYJDJC00090） |
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| 摘 要： | 近年来，毛细管电泳技术以其高效、微量、分离模式多样等优势，在生物大分子分析领域得到了日益广泛的应用.综述了毛细管电泳技术在多糖、蛋白质、核酸和脂质中的应用，并对其相关的样品制备、毛细管内壁吸附、仪器的研制等方面存在的问题进行分析和展望，以期为毛细管电泳技术在生物大分子领域的推广应用提供借鉴.
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| 关 键 词： | 毛细管电泳 多糖 蛋白质 核酸 脂质 |
| 收稿时间： | 2022-08-01 |
Application and Research Progress on Capillary Electrophoresis for Biological Macromolecular Analysis |
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| Affiliation: | 1.College of Traditional Chinese Medicine and Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China2.State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China |
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| Abstract: | The technology of capillary electrophoresis has been widely used in the field of biological macromolecular analysis in recent years because of its advantages such as high separation efficiency, small injection volume and diverse separation modes. The application of the capillary electrophoresis in the fields of biological macromolecules, such as polysaccharides, proteins, nucleic acids and lipids, were reviewed. And the problems related to this technology, such as sample preparation, capillary wall adsorption, instrument reproducibility and sensitivity, were analyzed and prospected. The purpose is to provide a reference for the application of the technology of capillary electrophoresis in the field of biological macromolecular analysis. |
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