| 金银花绿原酸提取方法研究 |
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| 引用本文： | 李辉,逯桃桃,陈佳,赵亮,邱洪灯. 金银花绿原酸提取方法研究[J]. 分析测试技术与仪器, 2022, 28(4): 416-421. DOI: 10.16495/j.1006-3757.2022.04.008 |
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| 作者姓名： | 李辉 逯桃桃 陈佳 赵亮 邱洪灯 |
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| 作者单位： | 1.中国科学院 兰州化学物理研究所，甘肃 兰州 730000 |
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| 基金项目： | 兰州化物所青年合作基金（HZJJ20-08）, 甘肃省青年科技基金计划（20JR10RA292），甘肃省高等学校创新基金（2021A-137） |
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| 摘 要： | 采用高效液相色谱法测定金银花中绿原酸的含量，通过热回流法优化金银花绿原酸提取条件并进行正交试验分析. 试验结果表明，金银花中绿原酸最佳提取条件为乙醇体积分数80%、提取温度100 ℃、料液比1∶20 （g/mL）、提取时间2 h时，绿原酸提取率为7.1%. 方法分析准确、操作简便、设备要求低，可用作金银花的质量控制和开发利用.
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| 关 键 词： | 金银花 绿原酸 高效液相色谱 提取 |
| 收稿时间： | 2022-11-02 |
Study on Extraction Method of Chlorogenic Acid from Honeysuckle |
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| Affiliation: | 1.Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China2.Lanzhou City University, Lanzhou 730070, China3.University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The content of chlorogenic acid in honeysuckle was determined by high performance liquid chromatography (HPLC), the extraction conditions of chlorogenic acid from honeysuckle were optimized using a thermal reflux method and the orthogonal test was carried out. The results showed that the best extraction conditions of chlorogenic acid from honeysuckle was ethanol with a volume fraction of 80%, a solid-liquid ratio of 1∶20 (g/mL), an extraction temperature of 100 ℃, an extraction time of 2 h, and the extraction rate of chlorogenic acid was 7.1%. The method can be used for quality control, development and utilization of honeysuckle with accurate analysis, simple operation and low equipment requirements. |
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